Chair of Biotechnology & Biophysics
Biocenter, University of Würzburg
Tel.: 0931 31 88687
Fax: 0931 31 84509
Single-molecule fluorescence spectroscopy and imaging, super-resolution microscopy
The extraordinary temporal and spatial precision of human infectious processes relies on the exact and specific interactions of host and pathogen. Research into these processes requires sophisticated analytical methods. The small size of most infectious agents (bacteria, viruses) and the subcellular compartments in the host requires imaging with a spatial resolution close to electron microscopy to obtain insights into the molecular structure and organization of host pathogen interactions. The project of Markus Sauer aims to develop refined localization microscopy methods (dSTORM and PALM) capable of providing quantitative information on the distributions and densities of molecules with unmatched spatial resolution. For example, the sub-cellular localization of bacterial proteins secreted by Chlamydia (Thomas Rudel), Salmonella (Jörg Vogel) and Campylobacter (Cynthia Sharma) and their interacting host proteins will be determined.