piwik-script

Intern
    GRK 2243 "Understanding Ubiquitylation: From Molecular Mechanisms to Disease"

    Publications

    Diebold M, Schönemann L, Eilers M, Sotriffer C, Schindelin H (2023). Crystal structure of a covalently linked Aurora-A–MYCN complex. J Struct Biol. doi: 10.1107/S2059798322011433.

    van gen Hassend Pia, Pottikkadavath A, Delto C, Kuhn M, Endres M, Schönemann L, Schindelin H (2023). RanBP9 controls the oligomeric state of CTLH complex assemblies. J Biol Chem. doi: 10.1016/j.jbc.2023.102869.

    Bothe S, Hänzelmann P, Böhler S, Kehrein J, Zehe M, Wiedemann C, Hellmich UA, Brenk R, Schindelin H, Sotriffer C (2022). Fragment screening using biolayer interferometry reveals ligands targeting the SHP-motif binding site of the AAA+ ATPase p97. Commun Chem. doi: 10.1038/s42004-022-00782-5.

    Solvie D, Baluapuri A, Uhl L, Fleischhauer D, Endres T, Papadopulos, Aziba A, Gaballa A, Mikicic I, Isaakova E, Giansanti C, Jansen J, Jungblut M, Klein T, Schülein-Völk C, Maric H, Doose S, Sauer M, Beli P, Rosenwald A, Dobbelstein M, Wolf E, Eilers M (2022). MYC multimers shield stalled replication forks from RNA polymerase. Nature. doi: 10.1038/s41586-022-05469-4.

    Truongvan N, Li S, Misra M, Kuhn M, Schindelin H (2022). Structures of UBA6 explain its dual specificity for ubiquitin and FAT10. Nat Commun 13, 4789. doi: 10.1038/s41467-022-32040-6.

    Prieto-Garcia C, Hartmann O, Reissland M, Braun F, Bozkurt S, Pahor N, Fuss C, Schirbel A, Schülein-Völk C, Buchberger A, Calzado Canale MA, Rosenfeldt M, Dikic I, Münch C, Diefenbacher ME (2022). USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K. Mol Oncol. doi: 10.1002/1878-0261.13217

    Papadopoulos D, Solvie D, Baluapuri A, Endres T, Ha SA, Herold S, Kalb J, Giansanti C, Schülein-Volk C, Ade CP, Schneider C, Gaballa A, Vos S, Fischer U, Dobbelstein M, Wolf E, Eilers M (2021). MYCN recruits the nuclear exosome complex to RNA polymerase II to prevent transcription-replication conflicts. Mol Cell. doi: 10.1016/j.molcel.2021.11.002.

    Nair RM, Seenivasan A, Liu B, Chen D, Lowe ED, Lorenz S (2021). Reconstitution and Structural Analysis of a HECT Ligase-Ubiquitin Complex via an Activity-Based Probe. ACS Chem Biol. doi: 10.1021/acschembio.1c00433.

    Narain A, Bhandare P, Adhikari B, Backes S, Eilers M, Dölken L, Schlosser A, Erhard F, Baluapuri A, Wolf E (2021). Targeted protein degradation reveals a direct role of SPT6 in RNAPII elongation and termination. Mol Cell. doi: 10.1016/j.molcel.2021.06.016.

    Orth B, Sander B, Möglich A, Diederichs K, Eilers M, Lorenz S (2021). Identification of an atypical interaction site in the BTB domain of the MYC-interacting zinc-finger protein 1. Structure. doi: 10.1016/j.str.2021.06.005.

    Tolay N, Buchberger A (2021). Comparative profiling of stress granule clearance reveals differential contributions of the ubiquitin system. Life Sci Alliance. doi: 10.26508/lsa.202000927.

    Hartmann O, Reissland M, Maier CR, Fischer T, Prieto-Garcia C, Baluapuri A, Schwarz J, Schmitz W, Garrido-Rodriguez M, Pahor N, Davies CC, Bassermann F, Orian A, Wolf E, Schulze A, Calzado MA, Rosenfeldt MT, Diefenbacher ME (2021). Implementation of CRISPR/Cas9 Genome Editing to Generate Murine Lung Cancer Models That Depict the Mutational Landscape of Human Disease. Front Cell Dev Biol. doi: 10.3389/fcell.2021.641618.

    Roeschert I, Poon E, Henssen AG, Dorado Garcia H, Gatti M, Giansanti C, Jamin Y, Ade CP, Gallant P, Schülein-Völk C, Beli P, Richards M, Rosenfeldt M, Altmeyer M, Anderson J, Eggert A, Dobbelstein M, Bayliss R, Chesler L, Büchel G, Eilers M (2021). Combined inhibition of Aurora-A and ATR kinase results in regression of MYCN-amplified neuroblastoma. Nat Cancer 2:312-326.

    Rudalska R, Harbig J, Snaebjornsson MT, Klotz S, Zwirner S, Taranets L, Heinzmann F, Kronenberger T, Forster M, Cui W, D’Artista L, Einig E, Hinterleitner M, Schmitz W, Dylawerska A, Kang T-W, Poso A, Rosenfeldt MT, Malek NP, Bitzer M, Laufer S, Pichler BJ, Popov N, Schulze A, Zender L, Dauch D (2021). LXRα activation and Raf inhibition trigger lethal lipotoxicity in liver cancer. Nat Cancer 2: 201-217. doi: 10.1038/s43018-020-00168-3.

    Endres T, Solvie D, Heidelberger JB, Andrioletti V, Baluapuri A, Ade CP, Muhar M, Eilers U, Vos SM, Cramer P, Zuber J, Beli P, Popov N, Wolf E, Gallant P, Eilers M (2021). Ubiquitylation of MYC couples transcription elongation with double-strand break repair at active promoters. Mol Cell 81:830-844.e13. doi: 10.1016/j.molcel.2020.12.035.

    Koch RD, Hörner EM, Münch N, Maier E, Kozjak-Pavlovic V (2020). Modulation of Host Cell Death and Lysis Are Required for the Release of Simkania negevensis. Front Cell Infect Microbiol 10:594932. doi 10.3389/fcimb.2020.594932.

    Liess AKL, Kucerova A,Schweimer K, Schlesinger D, Dybkov O, Urlaub H, Mansfeld J, Lorenz S (2020).  Dimerization regulates the human APC/C-associated ubiquitin-conjugating enzyme UBE2S. Sci Signal 13:eaba8208. doi: 10.1126/scisignal.aba8208.

    Kim RQ, Misra M, Alexis G, Tomašković I, Shin D, Schindelin H, Filippov DV, Ovaa H, Dikic I, van der Heden van Noort GJ (2020).  Development of ADPribosyl ubiquitin analogs to study enzymes involved in Legionella infection. Chemistry  PMID: 33075184. doi: 10.1002/chem.202004590.

    Adhikari B, Bozilovic J, Diebold M, Schwarz JD, Hofstetter J, Schröder M, Wanior M, Narain A, Vogt M, Dudvarski Stankovic N, Baluapuri A, Schönemann L, Eing L, Bhandare P, Kuster B, Schlosser A, Heinzlmeir S, Sotriffer C, Knapp S & Wolf E (2020). PROTAC-mediated degradation reveals a non-catalytic function of AURORA-A kinase. Nat Chem Biol 16:1179–1188. doi: 10.1038/s41589-020-00652-y.

    Prieto-Garcia C, Hartmann O, Reissland M, Fischer T, Maier CR, Rosenfeldt M, Schülein-Völk C, Klann K, Kalb R, Dikic I, Münch C, Diefenbacher ME (2020). Inhibition of USP28 overcomes Cisplatin-Resistance of Squamous Tumors by Suppression of the Fanconi Anemia Pathway. BioRxiv doi: 10.1101/2020.09.10.291278.

    Triki M, Rinaldi G, Planque M, Broekaert D, Winkelkotte AM, Maier CR,  Raman S, Vandekeere A, Van Elsen J, Orth MF, Grünewald TGP, Schulze A, Fendt SM (2020). mTOR Signaling and SREBP Activity Increase FADS2 Expression and Can Activate Sapienate Biosynthesis. Cell Rep 31: 107806. doi: 10.1016/j.celrep.2020.107806.

    Ries LK, Liess AKL, Feiler CG, Spratt DE, Lowe ED, Lorenz S (2020). Crystal structure of the catalytic C-lobe of the HECT-type ubiquitin ligase E6AP. Protein Sci 29:1550-1554. doi: 10.1002/pro.3832.

    Green JL, Wu Y, Encheva V, Lasonder E, Prommaban A, Kunzelmann S, Christodoulou E, Grainger M, Truongvan N, Bothe S, Sharma V, Song W, Pinzuti I, Uthaipibull C, Srichairatanakool S, Birault V, Langsley G, Schindelin H, Stieglitz B, Snijders AP, Holder AA (2020). Ubiquitin activation is essential for schizont maturation in Plasmodium falciparum blood-stage development. PLoS Pathog 16: e1008640. doi: 10.1371/journal.ppat.1008640.

    Peissert S, Sauer F, Grabarczyk DB, Braun C, Sander G, Poterszman A, Egly JM, Kuper J, Kisker C (2020). In TFIIH the Arch domain of XPD is mechanistically essential for transcription and DNA repair. Nat Commun 11:1667. doi: 10.1038/s41467-020-15241-9.

    Prieto‐Garcia C, Hartmann O, Reissland M, Braun F, Fischer T, Walz S, Schülein‐Völk C, Eilers U, Ade CP, Calzado MA, Orian A, Maric HM, Münch C, Rosenfeldt M, Eilers M, Diefenbacher ME (2020). Maintaining protein stability of ∆Np63 via USP28 is required by squamous cancer cells. EMBO Mol Med 12:e11101. doi: 10.15252/emmm.201911101.

    Kaymak I, Maier CR, Schmitz W, Campbell AD, Dankworth B, Ade CP, Walz S, Paauwe M, Kalogirou C, Marouf H, Rosenfeldt MT, Gay DM, McGregor GH, Sansom OJ, Schulze A (2020). Mevalonate Pathway Provides Ubiquinone to Maintain Pyrimidine Synthesis and Survival in p53-Deficient Cancer Cells Exposed to Metabolic Stress. Cancer Res 80:189-203.

    Cossa G, Roeschert I, Prinz F, Baluapuri A, Silveira Vidal R, Schülein-Völk C, Chang YC, Ade CP, Mastrobuoni G, Girard C, Wortmann L, Walz S, Lührmann R, Kempa S, Kuster B, Wolf E, Mumberg D, Eilers M (2020). Localized Inhibition of Protein Phosphatase 1 by NUAK1 Promotes Spliceosome Activity and Reveals a MYC-Sensitive Feedback Control of Transcription. Mol Cell 77:1322-1339.e1311. doi: 10.1016/j.molcel.2020.01.008.

    Beckwitt EC, Jang S, Carnaval Detweiler I, Kuper J, Sauer F, Simon N, Bretzler J, Watkins SC, Carell T, Kisker C, Van Houten B (2020). Single molecule analysis reveals monomeric XPA bends DNA and undergoes episodic linear diffusion during damage search. Nat Commun 11:1356. doi: 10.1038/s41467-020-15168-1.

    Auer D, Hügelschäffer SD, Fischer AB, Rudel T (2019). The chlamydial deubiquitinase Cdu1 supports recruitment of Golgi vesicles to the inclusion. Cell Microbiol 22:e13136. doi: 10.1111/cmi.13136.

    Sbiera S, Perez-Rivas LG, Taranets L, Weigand I, Flitsch J, Graf E, Monoranu CM, Saeger W, Hagel C, Honegger J, Assie G, Hermus AR, Stalla GK, Herterich S, Ronchi CL, Deutschbein T, Reincke M, Strom TM, Popov N, Theodoropoulou M, Fassnacht M (2019). Driver mutations in USP8 wild-type Cushing's disease. Neuro Oncol 21:1273-1283. doi: 10.1093/neuonc/noz109.

    Liess AKL, Kucerova A, Schweimer K, Yu L, Roumeliotis TI, Diebold M, Dybkov O, Sotriffer C, Urlaub H, Choudhary JS, Mansfeld J, Lorenz S (2019). Autoinhibition Mechanism of the Ubiquitin-Conjugating Enzyme UBE2S by Autoubiquitination. Structure 27:1195-1210.e7. doi: 10.1016/j.str.2019.05.008.

    Sauer F, Klemm T, Kollampally RB, Tessmer I, Nair RK, Popov N, Kisker C (2019). Differential Oligomerization of the Deubiquitinases USP25 and USP28 Regulates Their Activities. Mol Cell 74, 421-435.

    Deol KK, Lorenz S, Strieter ER (2019). Enzymatic Logic of Ubiquitin Chain Assembly. Front Physiol 10: 835. doi: 10.3389/fphys.2019.00835.

    Baluapuri A, Hofstetter J, Dudvarski Stankovic N, Endres T, Bhandare P, Vos SM, Adhikari B, Schwarz JD, Narain A, Vogt M, Wang SY, Düster R, Jung LA, Vanselow JT, Wiegering A, Geyer M, Maric HM, Gallant P, Walz S, Schlosser A, Cramer P, Eilers M, Wolf E (2019). MYC Recruits SPT5 to RNA Polymerase IIto Promote Processive Transcription Elongation. Mol Cell 74:674-687.

    Herold S, Kalb J, Büchel G, Ade CP, Baluapuri A, Xu J, Koster J, Solvie D, Carstensen A, Klotz C, Rodewald S, Schülein-Völk C, Dobbelstein M, Wolf E, Molenaar J, Versteeg R, Walz S, Eilers M (2019). Recruitment of BRCA1 limits MYCN-driven accumulation of stalled RNA polymerase. Nature 567:545–549.

    Ries LK, Sander B, Deol KK, Letzelter MA, Strieter ER, Lorenz S (2019). Analysis of ubiquitin recognition by the HECT ligase E6AP provides insight into its linkage specificity.  J Biol Chem. 294:6113-6129.

    Ramirez YA, Adler TB, Altmann E, Klemm T, Tiesmeyer C, Sauer F, Kathman SG, Statsyuk AV, Sotriffer C, Kisker C (2018). Front Cover: Structural Basis of Substrate Recognition and Covalent Inhibition of Cdu1 from Chlamydia trachomatis. ChemMedChem 13:2014–2023.

    Lorenz S (2018). Structural mechanisms of HECT-type ubiquitin ligases. Biol Chem 399:127-145. doi: 10.1515/hsz-2017-0184.

    Fischer A, Rudel T (2018). Safe haven under constant attack-The Chlamydia-containing vacuole. Cell Microbiol 20:e12940. doi: 10.1111/cmi.129400.

    Chen D, Gehringer M, Lorenz S. (2018). Developing small-molecule inhibitors of HECT-type ubiquitin ligases for therapeutic applications: challenges and opportunities. ChemBioChem 19:2123-2135.

    Turakhiya A, Meyer SR, Marincola G, Böhm S, Vanselow JT, Schlosser A, Hofmann K, Buchberger A (2018). ZFAND1 recruits p97 and the 26S proteasome to promote the clearance of arsenite-induced stress granules. Mol Cell 70:906-919.

    Wu Y, Pons V, Goudet A, Panigai L, Fischer A, Herweg JA, Kali S, Davey RA, Laporte J, Bouclier C, Yousfi R, Aubenque C, Merer G, Gobbo E, Lopez R, Gillet C, Cojean S, Popoff MR, Clayette P, Le Grand R, Boulogne C, Tordo N, Lemichez E, Loiseau PM, Rudel T, Sauvaire D, Cintrat JC, Gillet D, Barbier J (2017). ABMA, a small molecule that inhibits intracellular toxins and pathogens by interfering with late endosomal compartments. Sci Rep 7:15567. doi: 10.1038/s41598-017-15466-7.

    Misra M, Kuhn M, Löbel M, An H, Statsyuk AV, Sotriffer C, Schindelin H (2017). Dissecting the Specificity of Adenosyl Sulfamate Inhibitors Targeting the Ubiquitin-Activating Enzyme. Structure 25:1120-1129.e1123. doi: 10.1016/j.str.2017.05.001.

    Kaiser S, Sauer F, Kisker C (2017). The structural and functional characterization of human RecQ4 reveals insights into its helicase mechanism. Nat Commun 8:15907. doi: 10.1038/ncomms15907.

    Hänzelmann P, Schindelin H (2017). The Interplay of Cofactor Interactions and Post-translational Modifications in the Regulation of the AAA+ ATPase p97. Front Mol Biosci 4:21. doi: 10.3389/fmolb.2017.00021.

    Dejure FR, Royla N, Herold S, Kalb J, Walz S, Ade CP, Mastrobuoni G, Vanselow JT, Schlosser A, Wolf E, Kempa S, Eilers M (2017). The MYC mRNA 3'-UTR couples RNA polymerase II function to glutamine and ribonucleotide levels. Embo j 36:1854-1868. doi: 10.15252/embj.201796662.

    Büchel G, Carstensen A, Mak KY, Roeschert I, Leen E, Sumara O, Hofstetter J, Herold S, Kalb J, Baluapuri A, Poon E, Kwok C, Chesler L, Maric HM, Rickman DS, Wolf E, Bayliss R, Walz S, Eilers M (2017). Association with Aurora-A Controls N-MYC-Dependent Promoter Escape and Pause Release of RNA Polymerase II during the Cell Cycle. Cell Rep 21:3483-3497. doi: 10.1016/j.celrep.2017.11.090.