Deutsch Intern
    Rudolf Virchow Center for Integrative and Translational Bioimaging

    Visualizing specific structures based on selective degradation


    Visualizing specific structures over background fluorescence can be challenging. The Wehman lab has developed a labeling technique based on selective degradation that allows super-resolution insights on standard microscopes, improving the imaging of specific proteins, organelles, and cells in many model systems. The project has been published in Nature Communications.

    Part of Figure 1 [Comparison of degron approaches. a Proteins with a zinc finger 1 degron (ZF1, red) are stable before expression of the ubiquitin ligase adaptor ZIF-1 (blue) in C. elegans embryos.]

    Link to the publication: "Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures"  

    Katharina B. Beer, Gholamreza Fazeli, Kristyna Judasova, Linda Irmisch, Jona Causemann, Jörg Mansfeld & Ann M. Wehman 
    Nature Communications Volume 10, Article number: 3490 (2019)

    Link to Wehmann Lab Page