Equipment

Leica TCS SP8 Laser-Scanning-Microscope

Objectives	2.5x Dry 10x Dry, 20x IMM, 63x Oil, 63x W
Laser	405 nm, 440 nm, 458 nm, 476 nm, 488 nm, 496 nm, 514 nm, 561 nm, 594 nm, 633 nm
Light sources	HXP Fluorescent lamp, transmitted light
Fluorescence filter	AOBS (Acousto Optical Beam Splitter)
Detectors	3 PMTs, 2 HyD
Microscope stand	inverse
Special feature	Extension for light sheet microscopy with 5x and 10x objective lens
User model	Independent use
Costs	15,- €/h internal (Employees of the RVZ) 25,- €/h external
Contact person	Mike Friedrich Tel.: +49 931 31-80356 (office) E-Mail: mike.friedrich@uni-wuerzburg.de
Location	RVZ Building D15 / Room 00.109 Josef-Schneider-Str. 2 97080 Würzburg
Laboratory type	Safety level 1 laboratory

Zeiss LSM 980 Airyscan 2

Objectives	2,5x Dry, 10x Dry, 20x IMM, 40x IMM, 63x Oil
Laser	405 nm, 445 nm, 488 nm, 514 nm, 543 nm, 561 nm, 593 nm, 639 nm
Light sources	HBO Fluorescent lamp, transmitted light
Fluorescence filter	Spectrale Detection
Detectors	Internal 2 PMT, 32-channel spectral detector; External 2 PMT, 2 SPAD
Microscope stand	inverse
Special feature	rapidFLIM-Modul für FLIM, FCS and anisotropie
DFG device number	INST 93/1022-1
User model	Independent use
Costs	30,- €/h internal (Employees of the RVZ) 50,- €/h external
Contact person	Mike Friedrich Tel.: +49 931 31-80356 (office) E-Mail: mike.friedrich@uni-wuerzburg.de
Location	RVZ Building D15 / Room 00.107 Josef-Schneider-Str. 2 97080 Würzburg
Laboratory type	Safety level 1 laboratory

Leica TCS SP8 MP Multiphoton Microscope

Objectives	Revolver: 10x Dry, 40x W; Schieber: 25x W, 25x IMM
Laser	488 nm, Ti:Sa 680 - 1080 nm, OPO 1000 - 1340 nm
Light sources	HXP Fluorescent lamp, transmitted light
Fluorescence filter	Descanned: AOBS (Acousto Optical Beam Splitter), non-descanned: 4
Detectors	3 PMT, 2 HyD
Microscope stand	Upright
Special feature	Intravital microscopy
User model	Independent use
Costs of use	30,- €/h internal (Employees of the RVZ) 50,- €/h external
Contact persons	Univ.-Prof. Dr. med. A. Zernecke-Madsen Tel.: +49 931 31-83171 E-Mail: alma.zernecke@uni-wuerzburg.de Mike Friedrich Tel.: +49 931 31-80356 (office) E-Mail: mike.friedrich@uni-wuerzburg.de
Location	Building D16 / Room 2.202 Josef-Schneider-Str. 2 97080 Würzburg
Laboratory type	Safety level 1 laboratory

5x Light-sheet Fluorescence Microscope

Objectives	5x Dry
Laser	488 nm, 532 nm, 560 nm, 640 nm, 730 nm,
Light sources	none
Fluorescence filter	5
Detectors	sCMOS Andor Neo
User model	Independent use
Costs	15,- €/h internal (Employees of the RVZ) 25,- €/h external
Contact person	Mike Friedrich Tel.: +49 931 31-80356 (office) E-Mail: mike.friedrich@uni-wuerzburg.de
Location	RVZ Building D15 / Room 00.117 Josef-Schneider-Str. 2 97080 Würzburg
Laboratory type	Safety level 1 laboratory

20x Light-sheet Fluorescence Microscope

Objectives	20x IMM
Laser	488 nm, 532 nm, 560 nm, 640 nm, 730 nm,
Light sources	none
Fluorecence filter	5
Detectors	sCMOS Andor Neo
User model	Independent use
Costs of use	15,- €/h internal (Employees of the RVZ) 25,- €/h external
Contact person	Mike Friedrich Tel.: +49 931 31-80356 (Büro) E-Mail: mike.friedrich@uni-wuerzburg.de
Location	RVZ Building D15 / Room 00.117 Josef-Schneider-Str. 2 97080 Würzburg
Laboratory type	Safety level 1 laboratory

Leica DMI 6000 Fluorescence Microscope

Objectives	10 x Dry, 20 x Dry, 40 x Dry, 63 x Gly, 100 x Oil
Laser
Light sources	HBO Fluorescent lamp, transmitted light
Fluorescence filter	3
Detectors	sCMOS Leica
Microscope stand	inverse
Special feature	Live cell imaging with CO2 and temperature control
User model	Independent use
Costs of use	10,- €/h internal (Employees of the RVZ) 15,- €/h external
Contact person	Mike Friedrich Tel.: +49 931 31-80356 (office) E-Mail: mike.friedrich@uni-wuerzburg.de
Location	RVZ Building D15 / Room 00.108 Josef-Schneider-Str. 2 97080 Würzburg
Laboratory type	Safety level 1 laboratory

Zeiss Observer. Z1 dSTORM-Microscope

Objectives	10x Dry, 20x Dry, 40x Oil, 63x Oil, 63x W
Laser	532 nm, 640 nm
Light source	HBO Fluorescent lamp, transmitted light
Fluorescence filter	2
Detectors	EMCCD Andor iXon Ultra
Microscope stand	inverse
User model	Independent use
Costs of use	10,- €/h internal (Employees of the RVZ) 15,- €/h external
Location	RVZ Building D15 / Room 00.108 Josef-Schneider-Str. 2 97080 Würzburg
Laboratory type	Safety level 1 laboratory

PRIMO / Leica DMI 8 Fluorescence Microscope

Objectives	4 x Dry, 10 x Dry, 20 x Dry, 100 x Oil
Laser	405 nm
Light sources	LED5 Fluorescence source, transmitted light
Fluorescence filter	4
Detectors	sCMOS Leica
Microscope stand	Inverse
Special features	2D and 3D photo-coating and microfabrication with Leonardo software from Alveole
User model	Independent use
Costs of use	10,- €/h für Internal (Employees of RVZ) 15,- €/h für External
Contact person	Mike Friedrich Tel.: +49 931 31-80356 (Büro) E-Mail: mike.friedrich@uni-wuerzburg.de
Location	RVZ Building D15 / Room 00.104 Josef-Schneider-Str. 2 97080 Würzburg
Laboratory type	Safety laevel 1 laboratory

Nikon Eclipse Ti2-E Fluoreszenzmikroskop

Nikon Eclipse Ti2-E Fluorescence Microscope

Objectives	10x Dry, 20x Dry, (40x Long Distance Water), 60x Oil
Laser	n/a
Light sources	LED Fluorescence lamp 390, 440, 475, 510, 555, 575, 632, 740nm, transmitted light
Fluorescence filter	DAPI, FITS, TRITS, Cy5, Cy7; CFP, YFP, mCherry; TexasRED
Detectors	Hamamatsu Back Illuminated sCMOS
Microscope stand	inverse
Special features	Live cell imaging with CO2 and temperature control
DFG Gerätenummer	n/a
User model	Independent use
Costs of use	15,- €/h internal (Employees of RVZ)
Costs of use	25,- €/h external
Contact person	Mike Friedrich
	Tel.: +49 931 31-80356 (office)
	E-Mail: mike.friedrich@uni-wuerzburg.de

	Jürgen Pinnecker
	Tel.: +49 931 31-86800 (office)
	E-Mail: juergen.pinnecker@uni-wuerzburg.de

Location	RVZ
	Building D15 / Room 00.108
	Josef-Schneider-Str. 2
	97080 Würzburg
Laboratory type	Safety level 1 laboratory

Picoquant FT300 Spectrometer

Objectives	n/a
Laser	450 nm, 485 nm, 560 nm, 640 nm, 730 nm (all max. 80 Mhz)
Light sources	Xenon Lamp
Fluorescence filter	Spectral detection using monochromators
Detectors	PMA Hybrid
Special features	Lifetime spectrometer with Xe lamp for recording fluorescence excitation and emission spectra, anisotropy
User model	Independent use
Costs of use	0,- €/h internal (Employees of RVZ) 15,- €/h external
Contact	Dr. Katherina Hemmen Tel.: +49 931 31- 89875 (office) E-Mail: katherina.hemmen@uni-wuerzburg.de
Location	RVZ Building D15 / Room 00.104 Josef-Schneider-Str. 2 97080 Würzburg
Laborarory type	Safety level 1 laboratory

CUI-BEE / Workstation (16 cores, 1024 Gb RAM)

Processors	2 x Intel Xeon Gold 6244 3.6GHz,(4.4GHz) (8 Cores)
RAM	1024 GB
GPU	NVIDIA Quadro RTX6000, 24GB
Software/Licence	Imaris, Huygens, Matlab, Fiji, Ilastik
Special features	5 users simultaneously, remote access only, login details available on request
User model	Independent use
Costs of use	0,- €/h internal/external
Contact persons	Dr. Katherina Hemmen Tel.: +49 931 31- 89875 (office) E-Mail: katherina.hemmen@uni-wuerzburg.de Joachim Schenk Tel.: +49 931 31-87753 E-Mail: j oachim.schenk@uni-wuerzburg.de

Workstation 1 / License (24 cores, 256 GB RAM)

Processors	AMD EPYC 7443 24-Cores, 2.85 GHz
RAM	256 GB
GPU	Nvidia GeForce RTX 4060 Ti
Software/Licence	Imaris, Matlab, Fiji, Ilastik
Special features	Single-User, Remote-Zugriff possible, login details available on request
User model	Independent use
Costs of use	0,- €/h
Contact persons	Dr. Katherina Hemmen Tel.: +49 931 31- 89875 E-Mail: katherina.hemmen@uni-wuerzburg.de Joachim Schenk Tel.: +49 931 31-87753 E-Mail: joachim.schenk@uni-wuerzburg.de

Workstation 2 / License (24 cores, 320 Gb)

Processors	AMD EPYC 7443 24-Cores, 2.85 GHz
RAM	320 GB
GPU	Nvidia GeForce RTX 4060 Ti
Software / Licence	Imaris, Matlab, Fiji, Ilastik
Special features	Single user, remote access possible, login details available on request
usage model	Application mode
Costs of use	0,- €/h
Contact persons	Dr. Katherina Hemmen Tel.: +49 931 31- 89875 (Büro) E-Mail: katherina.hemmen@uni-wuerzburg.de Joachim Schenk Tel.: +49 931 31-87753 E-Mail: joachim.schenk@uni-wuerzburg.de

Software Huygens Deconvolution / License

Software/License:	Huygens
Special features:	Single-User-Licence, only on CUI-BEE
User model:	Independent use
Costs of use:	0,- €/h

Contact persons:

Dr. Katherina Hemmen
Tel.: +49 931 31- 89875
E-Mail: katherina.hemmen@uni-wuerzburg.de

Joachim Schenk
Tel.: +49 931 31-87753
E-Mail: joachim.schenk@uni-wuerzburg.de

Software Imaris / License

Software / License:		Bitplane Imaris
Special feature:		3x Single user license
Name	# Licenses	Used for?
Deprecated (InPress)	--	No longer used (deprecated by Imaris itself, only for very old versions)
FilamentTracer	2	Tracing of filamenteous structures, e.g. neurons or vessels
Imaris Cell	2	Segmentation of cellular structures which e.g. the nucleus and the cell membrane are stained
Imaris Coloc	2	Colocalization analysis
Imaris Lineage	2	To be used in combination with Imaris Track, can trac trace the lineage of dividing cells
Imaris Track	2	Tracking of objects
Imaris Vantage	1	To generate plots inside of Imaris, e.g. box plot of cell size distribution
Imaris XT	2	Ability to use extensions like direct data transfer to Fiji, python, Matlab or any of the (self-built) extensions. A list of XTensions can be found here: https://imaris.oxinst.com/open/
User model:		Independent use
Costs of use:		0,- €/h

Contact persons:

Dr. Katherina Hemmen
Tel.: +49 931 31- 89875
E-Mail: katherina.hemmen@uni-wuerzburg.de

Joachim Schenk
Tel.: +49 931 31-87753
E-Mail: joachim.schenk@uni-wuerzburg.de

Software Arivis / License

Software/License:		Bitplane Imaris
Special feature:		3x Single-User-License
Name	# Licenses	Used for?
Deprecated (InPress)	--	No longer used (deprecated by Imaris itself, only for very old versions)
FilamentTracer	2	Tracing of filamenteous structures, e.g. neurons or vessels
Imaris Cell	2	Segmentation of cellular structures which e.g. the nucleus and the cell membrane are stained
Imaris Coloc	2	Colocalization analysis
Imaris Lineage	2	To be used in combination with Imaris Track, can trac trace the lineage of dividing cells
Imaris Track	2	Tracking of objects
Imaris Vantage	1	To generate plots inside of Imaris, e.g. box plot of cell size distribution
Imaris XT	2	Ability to use extensions like direct data transfer to Fiji, python, Matlab or any of the (self-built) extensions. A list of XTensions can be found here: https://imaris.oxinst.com/open/
User model:		Independent use
Costs of use:		0,- €
Contact persons:		Dr. Katherina Hemmen Tel.: +49 931 31- 89875 (office) E-Mail: katherina.hemmen@uni-wuerzburg.de Joachim Schenk Tel.: +49 931 31-87753 E-Mail: j oachim.schenk@uni-wuerzburg.de

HeinzeHub (JupyterHub)

Processor:	AMD EPYC 7443P (24 Cores, 2.85GHz)
RAM:	320 GB

GPU:	NVIDIA A4500 (20GB), NVIDIA A5000 (24GB)
Special feature:	Remote access only, login details available on request
User model:	Independent use
Costs of use:	0,- €/h

Contact
persons:

Dr. Katherina Hemmen
Tel.: +49 931 31- 89875
E-Mail: katherina.hemmen@uni-wuerzburg.de

Joachim Schenk
Tel.: +49 931 31-87753
E-Mail: joachim.schenk@uni-wuerzburg.de

Full support during the booked microscopy or data analysis hour will incur an additional charge of € 25.00.

Picture credits